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sequence based reagent pgem t easy backbone promega rrid addgene 122563 commercial assay  (Addgene inc)


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    Structured Review

    Addgene inc sequence based reagent pgem t easy backbone promega rrid addgene 122563 commercial assay
    Sequence Based Reagent Pgem T Easy Backbone Promega Rrid Addgene 122563 Commercial Assay, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgem+t+easy+plasmid/pGEM-T+Easy-Gbait-hs-QF2+(Plasmid+%23122563)/10__7554_slash_elife__106439-335-33-41
    Average 93 stars, based on 3 article reviews
    sequence based reagent pgem t easy backbone promega rrid addgene 122563 commercial assay - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Cloning:

    Article Title: The receptor DNGR-1 signals for phagosomal rupture to promote cross-presentation of dead cell-associated antigens
    Article Snippet: .. The receptors were sub-cloned with the Takara In-Fusion Cloning system into the pSBbi-GH vector (Addgene) using the following primers: Primer Name Primer Sequence C7 forward CCCCAAGCTTGGCCTTTACAGTTCCTTCTCACAGA C7 reverse ACCCCAAGCTGGCCTATGAAATATCACTCTCATATAGA C9::C7 reverse ACCCCAAGCTGGCCTATGCATGCGGAAGAAATATATACC C9(Y7F)::C7 reverse ACCCCAAGCTGGCCTATGCATGCGGAAGAAATATTTACC Open in a separate window The murine β-2 microglobulin sequence in the pGEM-T Easy plasmid was obtained from Addgene (deposited by Peter Mombaerts) and sub-cloned with the Takara InFusion Cloning system into the pSBbi-bla plasmid (Addgene) using the following primers: Primer Name Primer Sequence β2M forward CCCCAAGCTTGGCCTAAAGCAGAAGTAGCCACAGGG β2M reverse ACCCCAAGCTGGCCTTTTCAGTGGCTGCTACTCGG Open in a separate window For the generation of stable lines. ..

    Plasmid Preparation:

    Article Title: The receptor DNGR-1 signals for phagosomal rupture to promote cross-presentation of dead cell-associated antigens
    Article Snippet: .. The receptors were sub-cloned with the Takara In-Fusion Cloning system into the pSBbi-GH vector (Addgene) using the following primers: Primer Name Primer Sequence C7 forward CCCCAAGCTTGGCCTTTACAGTTCCTTCTCACAGA C7 reverse ACCCCAAGCTGGCCTATGAAATATCACTCTCATATAGA C9::C7 reverse ACCCCAAGCTGGCCTATGCATGCGGAAGAAATATATACC C9(Y7F)::C7 reverse ACCCCAAGCTGGCCTATGCATGCGGAAGAAATATTTACC Open in a separate window The murine β-2 microglobulin sequence in the pGEM-T Easy plasmid was obtained from Addgene (deposited by Peter Mombaerts) and sub-cloned with the Takara InFusion Cloning system into the pSBbi-bla plasmid (Addgene) using the following primers: Primer Name Primer Sequence β2M forward CCCCAAGCTTGGCCTAAAGCAGAAGTAGCCACAGGG β2M reverse ACCCCAAGCTGGCCTTTTCAGTGGCTGCTACTCGG Open in a separate window For the generation of stable lines. ..

    Sequencing:

    Article Title: The receptor DNGR-1 signals for phagosomal rupture to promote cross-presentation of dead cell-associated antigens
    Article Snippet: .. The receptors were sub-cloned with the Takara In-Fusion Cloning system into the pSBbi-GH vector (Addgene) using the following primers: Primer Name Primer Sequence C7 forward CCCCAAGCTTGGCCTTTACAGTTCCTTCTCACAGA C7 reverse ACCCCAAGCTGGCCTATGAAATATCACTCTCATATAGA C9::C7 reverse ACCCCAAGCTGGCCTATGCATGCGGAAGAAATATATACC C9(Y7F)::C7 reverse ACCCCAAGCTGGCCTATGCATGCGGAAGAAATATTTACC Open in a separate window The murine β-2 microglobulin sequence in the pGEM-T Easy plasmid was obtained from Addgene (deposited by Peter Mombaerts) and sub-cloned with the Takara InFusion Cloning system into the pSBbi-bla plasmid (Addgene) using the following primers: Primer Name Primer Sequence β2M forward CCCCAAGCTTGGCCTAAAGCAGAAGTAGCCACAGGG β2M reverse ACCCCAAGCTGGCCTTTTCAGTGGCTGCTACTCGG Open in a separate window For the generation of stable lines. ..



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